Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Temperature control. Plate reader instructions Computer. Get Quote . It features a modular design that allows users to add or change detection cartridges according to their needs. Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today. All stand-alone instrument functions can be controlled using the software. 384-Deep Square Well Plates. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µL. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. 7°C and the bottom to 36. flat meniscus), but is approx 15 mL. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with. Dynamic range > 6 decades . 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. the use of a reader calibrated with primary NIST and NMI standards. Uncontrolled Copy for Training and Reference Purposes Only . After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. Reconstitution of reagents. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. Unparalleled performance on a personalized platform. SpectraMax i3x. plates < 100 amol (384-well) 1 0200-7019. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. Incubate the plates at room temperature for 20 min before reading plate luminescence using a Paradigm plate reader (Molecular Devices) (see Note 14). Page 1 of 8 . Catalog number: AB1384W. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. The third tested COC-based plate, the UltraVision 384-well microplate, however, absorbed at 2 OD at 200 nm,. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. Turn the reader on and secure the USB cable. After reading the ELISA plate on both readers, each standard curve was nearly identical. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Ideal for miniaturized 384 well fluorescent. More Product Information. x. M2. All microplate washers enable users to program tank selection, volumes, step times, and cycles for customized procedures. Molecular Devices, LLC. Cross-talk <0. x. These lab workhorses are used in drug discovery, research, bioassay validation, and. 384 Well Plates; Wavelength(s): 200 to 1000 nm (Abs), 250 - 850 nm (Fluor) Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. (40 µl maximum) Clear, white, or black. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. 6-384 (96- and 384-well with pipetting) 96-well microplates. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 0200-6262. Catalog number: BC1384W. High base plate design is compatible with automation and for bar coding. As the wells of this plate are closer together, the light is more likely to stray into adjacent wells. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as. Published Dec 16, 2020 | Updated Dec 16, 2020 Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum amount of DNA needed. 96- and 384-well plates; Wavelength(s): 635 nm (classification), 532 nm (reporter) Citations:. #3-000-510)Corning® 384 well microplate, low volume poly-D-lysine coated surface, black polystyrene, round bottom, sterile, lid, pack of 10; Synonyms: 384 multiwell plates,384 well low volume plates,384 well microplates,384 well microtiter plates,384 well plates,384 well tissue culture treated plates; find -CLS3643 MSDS, related peer-reviewed papers,. minutes and begin reading microplates with seven mouse clicks. x. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. Learn how the PathCheck feature can be applied to protein quantitation experiments using Molecular Devices absorbance-based microplate readers. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds, Monochromator, tunable 1. The microplate was then placed in a SpectraMax. 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Fluorescence Microplate Reader Comparison Chart. The plate reader optics are tunable for. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Molecular Devices 大中华区 Email: info. #ABS Plus). Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Shaking: Linear, orbital, and double orbital. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). 2 with 1000 cells. Efficiency. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. )Step 4: Incubate at room temperature for 1 hour, protected from light. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Streamline your workflow with comparative confluence assays to identify and verify gene edits. x. In simplest terms, a plate reader measures and. It also integrates with the. 58% for the detection module and 2. Packed 25 plates per sleeve, 2 sleeves per case. 96-1536. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modes <2 fg/well for firefly luciferase in 96- and 384- well top read. Ensure data accuracy of your reader. [Carbachol] (nM) RFU Max. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. X. Pro, Dual Read mode was selected. Read the microplate. Round well design with a solid black flat bottom2. • The FlexStation™ II instrument is designed specifically for 96-well microplates. Includes PC and software. This can increase throughput and efficiency, thus saving time and space in the lab. 25 µL/well in a 384-well plate, using AlphaScreen Phosphotyrosine (PT66) Assay Kit. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. Four wash/rinse bottles are included and configurable for both 96- and 384. ©2017 Molecular Devices, LLC . None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. 616, 665 nm EM filters 0200-7011. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. We would like to show you a description here but the site won’t allow us. The plate was also read at z height 1 mm with varying delay and integration times for the donor and acceptor reads. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 4 Equipment. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. 057, depending on lot number. Therefore, it is recommended that the pipettor height to be set to correspond to the fluid volume in the cell plate prior to compound addition. ,LTD (CAT#: STEM-S. Reader SpectraMax® Paradigm® Multi-Mode Microplate Reader. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. FlexIII. The FLIPR 384 system comes complete with 384. B. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Application Area: Multi mode plate reader. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Time-resolved phosphorescence. Microplate format. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). 5 ml microcentrifuge tubes 35 mm Petri dishes 12 mm × 50 mm test tubes (optional). Artel MVS Bundle DM Std/800TSNB Reader Details. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. 2. Microplates, cuvettes (via adapter) Detectors. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. Selects either 96-well or 384-well plate for a stand-alone read. 384-Well Microarray/High Sample Recovery Plate. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Supplier: Molecular Devices. x. For biotherapeutic development, the DynaPro Plate Reader is a comprehensive solution for stability evaluation. 1. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The GenePix® Microarray Systems are based on 25 years of expertise in low-noise signal amplification and optical design. 190. All stand-alone instrument functions can be controlled using the software. Embla washers are durable and easy to maintain. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. 1. Cells toxicity was corrected for the culture medium background absorbance. Related products. Summary of Contents for Molecular Devices SpectraMax M2. A = 2 - log 10 (%T). Select Start>Control panel>Device Manager. 384 well plate (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc. #R8190 (10) vials of component A*Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Molecular Devices Spectramax. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Plate Formats: 6- to 384-well plates. plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. The system provides automation for up to 50 microplates for easy walkaway automation. for the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. Rating: 3. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Ensure your assay quality with quality tips Certified RNase-/DNase-free Nonpyrogenic Nonsterile 50 racks/case. The analyzed results are. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. Microplate readers provide the ideal measurement platform for the high-throughput. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Assay setup for a 384-well low-volume plate. $402. x. easy to use and read multiple plates very quickly. 20 attomol ATP per well. and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. Ensure data accuracy of your reader. . Technical specifications. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). Add to Cart. Microplate is validated by molecular devices for the FLIPR reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. x. The FLIPR Fluorometric Imaging Plate Reader system addresses the industry's requirements for higher throughput, minimized sample volume, and cost reduction. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 96-1536. 7. VMax . A. Browse for different fluorescent readers below and compare across different instrument suppliers. X. Dual injectors for kinetic assays. Click SoftMax Pro Software User Guide. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates andThe Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. No accessories are needed and SoftMax® Pro Software. An industry-renowned instrument for monitoring GPCRs and ion channels. 384-well SBS microplates are designed with corners cut at a1 and h1 positions to conform to SBS nomenclature. UV and visible absorbanceLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. #3635) Transferpette micropipette (Drummond Scientific cat. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. in every plate. 2. Plate chamber design of the SpectraMax category 1 microplate readers. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. The Standard Thermo Scientific 384-well plate, Barcoded, White, is compatible with the majority of 384 block PCR and sequencing instruments. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. This application is highlighted by the example of a fully automated kinetic solubility screen in 384-well plate format. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The former have been the traditional domain of plate readers and great strides have been. 48, 96, and 384 wells can be used in the SpectraMax M2 and SpectraMax M2e. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. Embla washers are durable and easy to maintain. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Fluorescence Microplate Reader Comparison Chart. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. x. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 984. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. x. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). Method 1. Service parts for these models are not being produced and are no longer in stock. Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in HTS and lead optimization applications. 5 fg per well to 0. Detailed thermal cycling conditions are listed in Table 2. Optimizing settings in Standard 384 well black plate A standard black 384 well plate was set up as described in above Table 4. 2. SpectraMax iD5. 384-well TC plate, Poly-d-Lysine, Flat Clear. Versa Max. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates andCompany Molecular Devices, LLC; Price Get Quote; Catalog Number CSI / CSI FL; Type Cell Imaging System; Measuring Time White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min; Plate Formats 6, 12, 24, 96 & 384-well SBS microplates; Applications Cell Line Development, Fluorescence, Time-LapseNext-generation luminescence plate reader. Programmable 10% to 100%: Sample Cell Options: 96-, 384- or 1536-well microwell plates. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Get Quote . 48 ± 0. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. 76 ± 0. The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-Volume Sample Slide Figure 1-4. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. ) 0. the Gemini XPS reader. Important things to consider when purchasing a multimode microplate reader. Not for use in diagnostic procedures. 5 mP 1536–2. x. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. VIS absorbance detection. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Inquiry Basket. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. x. The screw remains inside the instrument. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. T = I/I 0 and %T = 100 (T). Efficient washing is achieved with cross-wise aspiration, reducing residual volume . Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. " A versatile UV/VIS spectrophotometer for both 96- and 384-well. 2. 0nm increments. 3% in white 96- and 384-well microplates Time. 00. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Figure 1. Guaranteed sensitivity: < 100 amol of biotinylated-LCK-P peptide, 25µL/well in a 384-well plate. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices'. Selects either 96-well or 384-well plate for a stand-alone read. Total volume (except deep. Dimensions 128 x 86 x 15 mm. and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wave-length range (spectral scan). Reading Capabilities: cuvette or microplate. CAPABILITIES OF THE EQUIPMENT: The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring. SpectraMax® ABS Plus microplate reader can run both cuvette-based and microplate reader applications on the same instrument. 96/384-well plate formats. Step 1: Open the SoftMax Pro IMAP FP protocol. ZERO BIAS - scores, article reviews, protocol conditions and more. 0 mP 384–1. were pipetted into a 96-well black-walled μclear plate. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. (96- and 384-well with pipetting) 6-384. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. DynaPro ™ Plate Reader III. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. The VICTOR Nivo. x. Top. All stand-alone instrument functions can be controlled using the software. EMax® Plus Microplate Reader, Molecular Devices Catalog Number: (10119-370) Supplier: Molecular Devices Description: Robust, high value microplate reader designed to. That explains the lower values on the LmaxII384 plate reader. 384- or 96-well read plate containing cells loaded. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 130009WThe SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. After the reaction was complete, 5 µL of each sample was transferred into wells of a 384-well plate and detected on the microplate readers using the instrument settings listed in Table 1. x. Temperature Control: ambient from +4 to 45C. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. For more sample throughput, the system canSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. The SpectraTest validation. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. 2. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. 5. x. Add 10 µL of ADP Detection Mix to those rows. 100 fmol luciferase per well. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. The Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. xABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. This reader provides visible absorbance, top-reading fluorescence intensity, and glow luminescence modes in 96- to 384-well plates with wavelength selection options from 340-650 nm. Related products. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. 1 a. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Shaking: Linear, orbital, and double orbital. Gemini EM Reader scans. use as little as 2 µL samples with 24- or 64-well plates. For more sample throughput, the system canThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. . 9. FLIPR Calcium 4 Assay Kit. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . Extended linear dynamic range. SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. For more sample throughput, the system can6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Published Dec 16, 2020 | Updated Nov 07, 2022. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. x. run on the FlexStation 3 reader in 384-well microplate with the FLIPR® Calcium 4 Assay Kit. 0 OD for earlier models. • 11/10 • Printed in USA • PN: 0120-1525. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Fluorescence Microplate Reader Comparison Chart. 046 to 0. Source plate capacity; 1 x plates. 2. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 6% for the on-board optics (monochromators). A control well was set up to verify the accuracy of the standard curve. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. The modular platform allows upgrading to expand functionality as your laboratory workflows change. flat-bottom: 50-60 µl. The. x. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. For fluorescence intensity assays, the SpectraMax® M2 and M2e units feature an. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Includes PC and software. Instrument pricing. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum well volume needed to run a 384 well plate? Minimum volume. 20 attomol ATP per well. Fluorescence Microplate Reader Comparison Chart. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. Figure 1. Tunable wavelength selection / monochromator. Two high-energy xenon flash lamps: Reading capabilities.